A Statistical Evaluation of Uncoupling Protein 1 in the Limited Area of Brown Adipose Tissue by Immunoelectron Microscopy

Uncoupling protein 1 (UCP1) expressed by the brown adipose tissue (BAT) in mitochondrial crista acts as a homeostatic thermogenerator of eutherians. The evaluation UCP1 expression BAT offers significant scientific insight, especially studies targeting limited areas such periarterial and pericardial regions small experimental mammals. However, negligible amount this would render general quantitative unreliable because lipid contamination low concentration. To address problem, we quantitatively evaluated mitochondrion mouse interscapular using immunoelectron microscopy immunohistochemical combination primary secondary antibodies scheme A (rabbit anti-UCP1 IgG/gold particle-conjugated goat anti-rabbit IgG), B C particle-unconjugated D IgG). Scheme shows immunopositive reaction obvious gold particles area, whereas other procedures revealed less distinctive reactions. particle immunoreaction comprised electrical high-density spots with mean diameter >5 nm. B, were scattered outside significantly smaller than 4 nm; schemes demonstrated few immunoreactions. Logistic regression analysis between showed that threshold measuring nm indicated true positive to antibody specifically area. Minor statistical difference was observed polyclonal IgG monoclonal antibodies. Therefore, might be useful for evaluating some areas, animals.